Study of Germin Like Oxalate Oxidase Enzyme in Monocot Plants
نویسندگان
چکیده
منابع مشابه
Germin, an Oxalate Oxidase, Has a Function in Many Aspects of Plant Life
Germin, a molecular marker of wheat embryo germination, is a remarkably proteaseresistant, apoplastic, homopentameric glycoprotein. Different forms of germin found to be related to various aspects of plant development have been discovered. Germins were determined in cereals, dicotyledonous angiosperms, gymnosperms and in a prosit, all these proteins are together called germin-like proteins due ...
متن کاملBarley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme.
Oxalate oxidase (EC 1.2.3.4) catalyses the conversion of oxalate and dioxygen into CO(2) and H(2)O(2). The barley (Hordeum vulgare) seedling root enzyme was purified to homogeneity and shown by metal analysis and EPR spectroscopy to contain Mn(II) at up to 0.80 atom per subunit. The involvement of Mn and neither flavin, Cu nor Fe in the direct conversion of dioxygen to H(2)O(2) makes oxalate ox...
متن کاملGermins and germin like proteins: an overview.
Molecular investigations during wheat germination have revealed unique developmentally regulated proteins, designated as germins, which show remarkable resistance to broad specificity proteases and to dissociation in SDS. Germins in cereals have an oxalate oxidase activity, which generates H2O2 from the oxidative breakdown of oxalate thereby playing a significant role in plant development and d...
متن کاملDetermination of urinary oxalate with commercially available oxalate oxidase.
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wid...
متن کاملAssay of plasma oxalate with soluble oxalate oxidase.
We use oxalate oxidase from barley seedlings for the colorimetric determination of oxalate in plasma. The oxalate is converted to hydrogen peroxide, which, in the presence of peroxidase, is detected by a Trinder-like chromogenic system. Optimization of the assay, including deproteinization and elimination of interferences from reducing substrates, is described. Ascorbate additions (200 mumol/L)...
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ژورنال
عنوان ژورنال: Indian Journal Of Agricultural Research
سال: 2019
ISSN: 0976-058X,0367-8245
DOI: 10.18805/ijare.a-5250